Journal: Journal of Radiation Research

Article Title: Low-dose energetic protons induce adaptive and bystander effects that protect human cells against DNA damage caused by a subsequent exposure to energetic iron ions

doi: 10.1093/jrr/rrv005

Figure Lengend Snippet: Proton-induced adaptive response in normal human cells. ( A ) Experiment schematic: Confluent normal human fibroblast cultures were first exposed to a priming radiation dose from energetic low-LET protons, followed 0, 6 or 24 h later by a challenge dose of high-LET iron ions. ( B ) Micronucleus formation (left panel) and micronuclei distribution (right panel) in AG1522 cells pre-irradiated with 20 cGy from 1-GeV protons ( 1 H) and challenged 0, 6 or 24 h later with 50 cGy of 1-GeV/u iron ions ( 56 Fe). ( C ) Micronucleus formation (left panel) and micronuclei distribution (right panel) in AG1522 cells pre-irradiated with 20 cGy from 0.05-GeV protons ( 1 H) and challenged 0, 6 or 24 h later with 50 cGy of 1-GeV/u iron ions ( 56 Fe). The data indicate that pre-exposure to a low dose of low-LET protons protects against DNA damage from a subsequent challenge from high-LET iron ions. * P < 0.05, ** P < 0.0001.

Article Snippet: Apparently normal human diploid skin fibroblasts (AG1522) were obtained from the Genetic Cell Repository at the Coriell Institute for Medical Research (Camden, NJ).

Techniques: Irradiation

Journal: Cell Death & Disease

Article Title: Characterization of novel markers of senescence and their prognostic potential in cancer

doi: 10.1038/cddis.2014.489

Figure Lengend Snippet: Expression and localization of senescence markers. Immunofluorescent images of selected targets in EJp16 and EJp21 uninduced (Control) or 4 days after tet removal (Senescent), as well as early passage IMR90 human fibroblasts compared with those entering replicative senescence after serial passaging. Nucleus are stained with DAPI (blue)

Article Snippet: IMR90 (human fibroblasts derived from lungs of a 16-week female foetus) and normal human diploid fibroblasts (Cellworks, San Jose, CA, USA) were maintained in DMEM supplemented with 10% FBS, and penicillin–streptomycin (50 unit/ml) until they reached replicative senescence.

Techniques: Expressing, Control, Passaging, Staining

Journal: Cell Death & Disease

Article Title: Characterization of novel markers of senescence and their prognostic potential in cancer

doi: 10.1038/cddis.2014.489

Figure Lengend Snippet: Defining a new FACS-based protocol for the detection of senescent cells. ( a ) Representative plot analysis of fluorescence levels in control and senescent EJp16, HT1080p21-9 and human diploid fibroblasts (HDF) stained with fluorescently tagged antibodies against B2MG, DEP1 and NOTCH3, as measured by flow cytometry. Senescent cells were analysed after 5 days of p16 or p21 expression. Numbers indicate mean fluorescent intensity (MFI) values. ( b ) Average fold increases of MFI of the same cells when senescence is induced. Experiments were performed in triplicate. Error bars show S.D.

Article Snippet: IMR90 (human fibroblasts derived from lungs of a 16-week female foetus) and normal human diploid fibroblasts (Cellworks, San Jose, CA, USA) were maintained in DMEM supplemented with 10% FBS, and penicillin–streptomycin (50 unit/ml) until they reached replicative senescence.

Techniques: Fluorescence, Control, Staining, Flow Cytometry, Expressing